Enzymatic Platforms for Sensitive Neurotransmitter Detection
Open Access
- 10 January 2020
- Vol. 20 (2), 423
- https://doi.org/10.3390/s20020423
Abstract
A convenient electrochemical sensing pathway was investigated for neurotransmitter detection based on newly synthesized silole derivatives and laccase/horseradish-peroxidase-modified platinum (Pt)/gold (Au) electrodes. The miniature neurotransmitter’s biosensors were designed and constructed via the immobilization of laccase in an electroactive layer of the Pt electrode coated with poly(2,6-bis(3,4-ethylenedioxythiophene)-4-methyl-4-octyl-dithienosilole) and laccase for serotonin (5-HT) detection, and a Au electrode modified with the electroconducting polymer poly(2,6-bis(selenophen-2-yl)-4-methyl-4-octyl-dithienosilole), along with horseradish peroxidase (HRP), for dopamine (DA) monitoring. These sensing arrangements utilized the catalytic oxidation of neurotransmitters to reactive quinone derivatives (the oxidation process was provided in the enzymes’ presence). Under the optimized conditions, the analytical performance demonstrated a convenient degree of sensitivity: 0.0369 and 0.0256 μA mM−1 cm−2, selectivity in a broad linear range (0.1–200) × 10−6 M) with detection limits of ≈48 and ≈73 nM (for the serotonin and dopamine biosensors, respectively). Moreover, the method was successfully applied for neurotransmitter determination in the presence of interfering compounds (ascorbic acid, L-cysteine, and uric acid).Keywords
This publication has 90 references indexed in Scilit:
- Detection and Quantification of Neurotransmitters in DialysatesCurrent Protocols in Neuroscience, 2009
- Overview of Brain MicrodialysisCurrent Protocols in Neuroscience, 2009
- Neuronal Source of Plasma DopamineClinical Chemistry, 2008
- Plasma serotonin levels and the platelet serotonin transporterJournal of Neurochemistry, 2007
- Overoxidized polypyrrole film directed DNA immobilization for construction of electrochemical micro-biosensors and simultaneous determination of serotonin and dopamineAnalytica Chimica Acta, 2005
- Post-column terbium complexation and sensitized fluorescence detection for the determination of norepinephrine, epinephrine and dopamine using high-performance liquid chromatographyAnalytica Chimica Acta, 2002
- A study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stabilityBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
- Oxidation of Phenols, Anilines, and Benzenethiols by Fungal Laccases: Correlation between Activity and Redox Potentials as Well as Halide InhibitionBiochemistry, 1996
- Multicopper Oxidases and OxygenasesChemical Reviews, 1996
- Serotonin in body fluids: Characterization of human plasmatic and cerebrospinal fluid pools by means of a new HPLC methodLife Sciences, 1985