HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity
Open Access
- 22 January 2021
- Vol. 13 (2), 171
- https://doi.org/10.3390/v13020171
Abstract
The ability to efficiently establish a new infection is a critical property for human immunodeficiency virus type 1 (HIV-1). Although the envelope protein of the virus plays an essential role in receptor binding and internalization of the infecting virus, the structural proteins, the polymerase and the assembly of new virions may also play a role in establishing and spreading viral infection in a new host. We examined Ugandan viruses from newly infected patients and focused on the contribution of the Gag-Pol genes to replication capacity. A panel of Gag-Pol sequences generated using single genome amplification from incident HIV-1 infections were cloned into a common HIV-1 NL4.3 pol/env backbone and the influence of Gag-Pol changes on replication capacity was monitored. Using a novel protein domain approach, we then documented diversity in the functional protein domains across the Gag-Pol region and identified differences in the Gag-p6 domain that were frequently associated with higher in vitro replication.Keywords
Funding Information
- International AIDS Vaccine Initiative (12345)
This publication has 56 references indexed in Scilit:
- Disease progression by infecting HIV-1 subtype in a seroconverter cohort in sub-Saharan AfricaAIDS, 2013
- HIV-1 Viral Subtype Differences in the Rate of CD4+ T-Cell Decline Among HIV Seroincident Antiretroviral Naive Persons in Rakai District, UgandaJAIDS Journal of Acquired Immune Deficiency Syndromes, 2010
- Replicative Capacity of Human Immunodeficiency Virus Type 1 Transmitted from Mother to Child Is Associated with Pediatric Disease Progression RateJournal of Virology, 2010
- CCR5- and CXCR4-Tropic Subtype C Human Immunodeficiency Virus Type 1 Isolates Have a Lower Level of Pathogenic Fitness than Other Dominant Group M Subtypes: Implications for the EpidemicJournal of Virology, 2009
- Genetic identity, biological phenotype, and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infectionThe Journal of Experimental Medicine, 2009
- jpHMM: Improving the reliability of recombination prediction in HIV-1Nucleic Acids Research, 2009
- Transmission of HIV-1 Gag immune escape mutations is associated with reduced viral load in linked recipientsThe Journal of Experimental Medicine, 2008
- Escape and Compensation from Early HLA-B57-Mediated Cytotoxic T-Lymphocyte Pressure on Human Immunodeficiency Virus Type 1 Gag Alter Capsid Interactions with Cyclophilin AJournal of Virology, 2007
- Coreceptor Tropism in Human Immunodeficiency Virus Type 1 Subtype D: High Prevalence of CXCR4 Tropism and Heterogeneous Composition of Viral PopulationsJournal of Virology, 2007
- WebLogo: A Sequence Logo Generator: Figure 1Genome Research, 2004