Mutations in the predicted DNA polymerase subunit POLD3 result in more rapid flowering of Brachypodium distachyon
- 31 August 2020
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 227 (6), 1725-1735
- https://doi.org/10.1111/nph.16546
Abstract
The timing of reproduction is a critical developmental decision in the life cycle of many plant species. Fine mapping of a rapid-flowering mutant was done using whole-genome sequence data from bulked DNA from a segregating F2 mapping populations. The causative mutation maps to a gene orthologous with the third subunit of DNA polymerase delta (POLD3), a previously uncharacterized gene in plants. Expression analyses of POLD3 were conducted via real time qPCR to determine when and in what tissues the gene is expressed. To better understand the molecular basis of the rapid-flowering phenotype, transcriptomic analyses were conducted in the mutant vs wild-type. Consistent with the rapid-flowering mutant phenotype, a range of genes involved in floral induction and flower development are upregulated in the mutant. Our results provide the first characterization of the developmental and gene expression phenotypes that result from a lesion in POLD3 in plants.Keywords
Funding Information
- National Science Foundation (IOS‐1258126, IOS‐1755224)
This publication has 45 references indexed in Scilit:
- A TILLING Platform for Functional Genomics in Brachypodium distachyonPLOS ONE, 2013
- Wheat TILLING Mutants Show That the Vernalization Gene VRN1 Down-Regulates the Flowering Repressor VRN2 in Leaves but Is Not Essential for FloweringPLoS Genetics, 2012
- REVIGO Summarizes and Visualizes Long Lists of Gene Ontology TermsPLOS ONE, 2011
- Characterization of the maintained vegetative phase deletions from diploid wheat and their effect on VRN2 and FT transcript levelsMolecular Genetics and Genomics, 2010
- Replication Stress Leads to Genome Instabilities inArabidopsisDNA Polymerase Δ MutantsPlant Cell, 2009
- Vernalization-induced flowering in cereals is associated with changes in histone methylation at the VERNALIZATION1 geneProceedings of the National Academy of Sciences, 2009
- Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsisPlant Cell, 2009
- DNA Polymerase δ Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA ExtensionMolecular and Cellular Biology, 2008
- INCURVATA2 Encodes the Catalytic Subunit of DNA Polymerase α and Interacts with Genes Involved in Chromatin-Mediated Cellular Memory in Arabidopsis thalianaPlant Cell, 2007
- Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and RicePlant Physiology, 2007