Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines
- 18 January 2021
- journal article
- research article
- Published by Wiley in Journal of Food Science
- Vol. 86 (2), 587-601
- https://doi.org/10.1111/1750-3841.15591
Abstract
Cancer is a noncommunicable disease of rising worldwide concern. Marine food products such as Octopus vulgaris ink (OI) could be sources of compounds addressing these concerns. This study aimed to evaluate the antimutagenic, cytoprotective, antiproliferative, proapoptotic, and antioxidant capacity of OI extracts on human cancer cell lines (22Rv1, HeLa, A549). The ARPE‐19 cell line was used as a reference human cell line to evaluate the ink's cytotoxicity. The water extract exhibited the highest antimutagenic and cytoprotective effect, but the dichloromethane extract (DM) showed the lowest half lethal concentration against 22Rv1 cells. Structural elucidation of purified DM fractions (F1, F2, F3) identified an unreported compound, N‐(2‐ozoazepan‐3‐yl)‐pyrrolidine‐2‐carboxamide (OPC). DM‐F2 showed high antiproliferative effect (LC50 = 27.6 µg/mL), reactive species modulation, early‐apoptosis induction (42.9%), and nuclei disruption in 22Rv1 cells. In silico analysis predicted high OPC affinity with Cyclin D1 (−6.70 kcal/mol), suggesting its potential impact on cell cycle arrest. These results highlight the antimutagenic, cytoprotective, and antiproliferative potential health benefits derived from underutilized marine food products such as OI. Further investigations at in vitro or in vivo levels are required to elucidate mechanisms and health benefits from OI. Practical Application O. vulgaris ink is an underutilized marine natural product that could be a source of biological compounds with potential health benefits such as antioxidant activity and cancer prevention.Keywords
Funding Information
- Consejo Nacional de Ciencia y Tecnología (2174, 241133)
This publication has 57 references indexed in Scilit:
- Shrimp Lipids: A Source of Cancer Chemopreventive CompoundsMarine Drugs, 2013
- Sepia Ink Oligopeptide Induces Apoptosis in Prostate Cancer Cell Lines via Caspase-3 Activation and Elevation of Bax/Bcl-2 RatioMarine Drugs, 2012
- Dynamics of H2O2 availability to ARPE-19 cultures in models of oxidative stressFree Radical Biology & Medicine, 2010
- AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreadingJournal of Computational Chemistry, 2009
- p65/RelA Modulates BECN1 Transcription and AutophagyMolecular and Cellular Biology, 2009
- Protective Effects of Squid Ink Extract Towards Hemopoietic Injuries Induced by CyclophosphamineMarine Drugs, 2009
- Colorimetric and highly selective “turn-on” fluorescent anion chemosensors with excited state proton transferJournal of Photochemistry and Photobiology A: Chemistry, 2007
- p27 Phosphorylation by Src Regulates Inhibition of Cyclin E-Cdk2Cell, 2007
- The regulation of cyclin D1 degradation: roles in cancer development and the potential for therapeutic inventionMolecular Cancer, 2007
- Studies on biological activities of melanin from marine animals. II. Purification of melanin from Octopus vulgaris Cuvier and its inhibitory activity on gastric juice secretion in rats.CHEMICAL & PHARMACEUTICAL BULLETIN, 1982