Metabolomic profiling of wild‐type and mutant soybean root nodules using laser‐ablation electrospray ionization mass spectrometry reveals altered metabolism
- 15 May 2020
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 103 (5), 1937-1958
- https://doi.org/10.1111/tpj.14815
Abstract
The establishment of the nitrogen-fixing symbiosis between soybean and Bradyrhizobium japonicum is a complex process. To document the changes in plant metabolism as a result of symbiosis, we utilized laser ablation electrospray ionization-mass spectrometry (LAESI-MS) for in situ metabolic profiling of wild-type nodules, nodules infected with a B. japonicum nifH mutant unable to fix nitrogen, nodules doubly infected by both strains, and nodules formed on plants mutated in the stearoyl-acyl carrier protein desaturase (sacpd-c) gene, which were previously shown to have an altered nodule ultrastructure. The results showed that the relative abundance of fatty acids, purines, and lipids was significantly changed in response to the symbiosis. The nifH mutant nodules had elevated levels of jasmonic acid, correlating with signs of nitrogen deprivation. Nodules resulting from the mixed inoculant displayed similar, overlapping metabolic distributions within the sectors of effective (fix+) and ineffective (nifH mutant, fix−) endosymbionts. These data are inconsistent with the notion that plant sanctioning is cell autonomous. Nodules lacking sacpd-c displayed an elevation of soyasaponins and organic acids in the central necrotic regions. The present study demonstrates the utility of LAESI-MS for high-throughput screening of plant phenotypes. Overall, nodules disrupted in the symbiosis were elevated in metabolites related to plant defense.Keywords
Funding Information
- National Science Foundation (IoS‐1734145)
- Biological and Environmental Research (DOE‐FOA‐0001192)
- U.S. Department of Energy
- University of Missouri
This publication has 111 references indexed in Scilit:
- Phenotypic and Genomic Analyses of a Fast Neutron Mutant Population Resource in SoybeanPlant Physiology, 2011
- Failure to fix nitrogen by non-reproductive symbiotic rhizobia triggers host sanctions that reduce fitness of their reproductive clonematesProceedings Of The Royal Society B-Biological Sciences, 2011
- Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicumPlant Physiology, 2010
- Differentiation of Symbiotic Cells and Endosymbionts in Medicago truncatula Nodulation Are Coupled to Two Transcriptome-SwitchesPLOS ONE, 2010
- Role of Gluconic Acid Production in the Regulation of Biocontrol Traits of Pseudomonas fluorescens CHA0Applied and Environmental Microbiology, 2009
- Coordinating Nodule Morphogenesis with Rhizobial Infection in LegumesAnnual Review of Plant Biology, 2008
- Medicago truncatulaRoot Nodule Proteome Analysis Reveals Differential Plant and Bacteroid Responses to Drought StressPlant Physiology, 2007
- Jasmonates: An Update on Biosynthesis, Signal Transduction and Action in Plant Stress Response, Growth and DevelopmentAnnals of Botany, 2007
- A Novel Ankyrin-Repeat Membrane Protein, IGN1, Is Required for Persistence of Nitrogen-Fixing Symbiosis in Root Nodules of Lotus japonicusPlant Physiology, 2007
- Determination of leghemoglobin components and xylem sap composition by capillary electrophoresis in hypernodulation soybean mutants cultivated in the fieldSoil Science and Plant Nutrition, 1998