Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents
- 1 October 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 192 (2), 573-584
- https://doi.org/10.1007/s12010-020-03345-3
Abstract
Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide. The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%). Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces. In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.Keywords
Funding Information
- National Natural Science Foundation of China (21908070, 21676104, 21878105)
- National Basic Research Program of China (2018YFC1603400, 2018YFC1602100)
- Postdoctoral Research Foundation of China (BX20180102, 2019M652902)
- Fundamental Research Funds for the Central Universities (2019MS100, 2019PY15)
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