The Future of Anticancer Drugs: A Cytotoxicity Assessment Study of CdSe/ZnS Quantum Dots
Open Access
- 20 August 2020
- journal article
- research article
- Published by MDPI AG in Journal of Nanotheranostics
- Vol. 1 (1), 19-38
- https://doi.org/10.3390/jnt1010003
Abstract
Quantum dots (QDs), including CdSe/ZnS, are nanoparticles emitting various wavelengths of fluorescent light depending on their size. Fluorescence allows them to be exploited for in vivo sensing/imaging of cancer cells. Nevertheless, thorough assessments of the effects of these commonly used QDs on cell stability are essentially required prior to their full applications. To investigate the effects of Cd QDs on the growth of human cervical cancer cells (HeLa), we utilized a growth assay, a reactive oxygen species (ROS) assay, an apoptosis assay, and RNA-seq. The growth assay results showed significant proliferation inhibition of HeLa cells by CdSe/ZnS. We revealed that smaller green CdSe/ZnS exerts more toxic effects than slightly larger yellow CdSe/ZnS. There were no significant increases of ROSs under the treatment of Cd QDs, which is consistent with the notion that low concentration of Cd QDs does not cause significant production of ROSs. In addition, we found that Cd QDs induced late apoptosis. RNA-Seq-based transcriptome analysis revealed that the exposure to green Cd QDs significantly upregulated antiapoptotic, antiproliferative, and antitumorigenic functions. The transcriptome profile also noted the downregulation of pro-proliferation, mitochondrial respiratory chain, detoxification, and receptor-mediated endocytosis. Taken together, our findings provide evidence that green CdSe/ZnS can be an alternative anticancer drug. In addition, our transcriptome analysis provides new insights into alteration of physiological state induced by CdSe/ZnS QDs in HeLa cancer cells.Keywords
Funding Information
- Engineer Research and Development Center (W912HZ19C0048)
This publication has 63 references indexed in Scilit:
- Understanding mitochondrial complex I assembly in health and diseaseBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2012
- Sprouty4 Is an Endogenous Negative Modulator of TrkA Signaling and Neuronal Differentiation Induced by NGFPLOS ONE, 2012
- Cellular uptake mechanisms and toxicity of quantum dots in dendritic cellsNanomedicine, 2011
- Targeting the Insulin-Like Growth Factor Pathway in Rhabdomyosarcomas: Rationale and Future PerspectivesSarcoma, 2011
- Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2Nature Genetics, 2009
- Toxicity of CdSe Nanoparticles in Caco-2 Cell CulturesJournal of Nanobiotechnology, 2008
- Long term predictive values of cytology and human papillomavirus testing in cervical cancer screening: joint European cohort studyBMJ, 2008
- Unmodified CdSe Quantum Dots Induce Elevation of Cytoplasmic Calcium Levels and Impairment of Functional Properties of Sodium Channels in Rat Primary Cultured Hippocampal NeuronsEnvironmental Health Perspectives, 2008
- SOD1 overexpression alters ROS production and reduces neurotoxic inflammatory signaling in microglial cellsJournal of Neuroimmunology, 2006
- Cytotoxicity of Colloidal CdSe and CdSe/ZnS NanoparticlesNano Letters, 2004