Apolipoprotein-mediated regulation of lipid metabolism induces distinctive effects in different types of breast cancer cells
Open Access
- 22 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Breast Cancer Research
- Vol. 22 (1), 1-17
- https://doi.org/10.1186/s13058-020-01276-9
Abstract
The highest incidence of breast cancer is in the Western world. Several aspects of the Western lifestyle are known risk factors for breast cancer. In particular, previous studies have shown that cholesterol levels can play an important role in the regulation of tumor progression. In the present study, we modulated cholesterol metabolism in the human breast cancer cell lines MCF-7 and MDA-MB-231 using a genetic approach. Apolipoprotein A-I (apoA-I) and apolipoprotein E (apoE) were expressed in these cell lines to modulate cholesterol metabolism. The effects of these apolipoproteins on cancer cell properties were examined. Our results show that both apolipoproteins can regulate cholesterol metabolism and can control the epithelial-to-mesenchymal transition process. However, these effects were different depending on the cell type. We show that expressing apoA-I or apoE stimulates proliferation, migration, and tumor growth of MCF-7 cells. However, apoA-I or apoE reduces proliferation and migration of MDA-MB-231 cells. These data suggest that modulating sterol metabolism may be most effective at limiting tumor progression in models of triple-negative cancers.Keywords
Funding Information
- Ligue Contre le Cancer (N/A)
- Conseil Régional du Centre-Val de Loire (N/A)
This publication has 46 references indexed in Scilit:
- Plant Sterols as Anticancer Nutrients: Evidence for Their Role in Breast CancerNutrients, 2013
- bc-GenExMiner 3.0: new mining module computes breast cancer gene expression correlation analysesDatabase: The Journal of Biological Databases and Curation, 2013
- CellMiner: A Web-Based Suite of Genomic and Pharmacologic Tools to Explore Transcript and Drug Patterns in the NCI-60 Cell Line SetCancer Research, 2012
- CAVEOLIN-1: Role in Cell SignalingAdvances in Experimental Medicine and Biology, 2012
- Role of Cholesterol in the Development and Progression of Breast CancerThe American Journal of Pathology, 2011
- Epithelial-Mesenchymal Transition in Cancer: Parallels Between Normal Development and Tumor ProgressionJournal of Mammary Gland Biology and Neoplasia, 2010
- CellMiner: a relational database and query tool for the NCI-60 cancer cell linesBMC Genomics, 2009
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implicationsProceedings of the National Academy of Sciences, 2001
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959