Phytoene synthase 1 (Psy-1) and lipoxygenase 1 (Lpx-1) Genes Influence on Semolina Yellowness in Wheat Mediterranean Germplasm
Open Access
- 29 June 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (13), 4669
- https://doi.org/10.3390/ijms21134669
Abstract
Phytoene synthase 1 (Psy1) and lipoxygenase 1 (Lpx-1) are key genes involved in the synthesis and catalysis of carotenoid pigments in durum wheat, regulating the increase and decrease in these compounds, respectively, resulting in the distinct yellow color of semolina and pasta. Here, we reported new haplotype variants and/or allele combinations of these two genes significantly affecting yellow pigment content in grain and semolina through their effect on carotenoid pigments. To reach the purpose of this work, three complementary approaches were undertaken: the identification of QTLs associated to carotenoid content on a recombinant inbred line (RIL) population, the characterization of a Mediterranean panel of accessions for Psy1 and Lpx-1 genes, and monitoring the expression of Psy1 and Lpx-1 genes during grain filling on two genotypes with contrasting yellow pigments. Our data suggest that Psy1 plays a major role during grain development, contributing to semolina yellowness, and Lpx-1 appears to be more predominant at post-harvest stages and during pasta making.Keywords
This publication has 54 references indexed in Scilit:
- Can Mediterranean durum wheat landraces contribute to improved grain quality attributes in modern cultivars?Euphytica, 2011
- Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grainsBMC Plant Biology, 2010
- Identification of suitable reference genes for normalization of qPCR data in comparative transcriptomics analyses in the TriticeaePlanta, 2010
- Allelic Variants at the Psy‐A1 and Psy‐B1 Loci in Durum Wheat and Their Associations with Grain YellownessCrop Science, 2009
- Identification and validation of reference genes for quantitative RT-PCR normalization in wheatBMC Molecular Biology, 2009
- Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre‐harvest sprouting and photo‐oxidation in riceThe Plant Journal, 2008
- Phytochemical Profiles and Antioxidant Activity of Wheat VarietiesJournal of Agricultural and Food Chemistry, 2003
- Effects of Modified Processing Conditions on Oxidative Properties of Semolina Dough and PastaCereal Chemistry Journal, 2003
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Durum Wheat Lipoxygenase Activity and Other Quality Parameters that Affect Pasta ColorCereal Chemistry Journal, 1999