Potential for the Biodegradation of Atrazine Using Leaf Litter Fungi from a Subtropical Protection Area
- 1 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Current Microbiology
- Vol. 78 (1), 358-368
- https://doi.org/10.1007/s00284-020-02288-6
Abstract
The intense use of pesticides in agricultural activities for the last several decades has caused contamination of the ecosystems connected with crop fields. Despite the well-documented occurrence of pesticide biodegradation by microbes, natural attenuation of atrazine (ATZ), and its effects on ecological processes in subtropical forested areas, such as Iguacu National Park located in Brazil, has been poorly investigated. Subtropical environments sustain a great degree of fungal biodiversity, and the patterns and roles of these organisms should be better understood. This work aimed to evaluate nine ligninolytic-producer fungi isolated from the INP edge to degrade and detoxify ATZ solutions. ATZ degradation and the main metabolites produced, including deisopropylatrazine and deethylatrazine (DEA), were analyzed using dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometer. Four fungi were able to degrade ATZ to DEA, and the other five showed potential to grow and facilitate ATZ biodegradation. Furthermore, two strains of Fusarium spp. showed an enhanced potential for detoxification according to the Allium cepa (onion) test. Although the isolates produced ligninolytic enzymes, no ligninolytic activity was observed in the biodegradation of ATZ, a feature with ecological significance. In conclusion, Ascomycota fungi from the INP edge can degrade and detoxify ATZ in solution. Increasing the knowledge of biodiversity in subtropical protected areas, such as ecosystem services provided by microbes, enhances ecosystem conservation.Funding Information
- CNPq (406563/2016-0)
- UNILA
This publication has 49 references indexed in Scilit:
- The Fungi: 1, 2, 3 … 5.1 million species?American Journal of Botany, 2011
- Allium cepa test in environmental monitoring: A review on its applicationMutation Research, 2009
- Microbial adaptation for accelerated atrazine mineralization/ degradation in Mississippi Delta soilsWeed Science, 2006
- Complete Nucleotide Sequence and Organization of the Atrazine Catabolic Plasmid pADP-1 from Pseudomonas sp . Strain ADPJournal of Bacteriology, 2001
- Teaching techniques for mycology: 11. Riddell's slide culturesMycologist, 2000
- Discordant Groupings of Fusarium spp. from Sections Elegans, Liseola and Dlaminia Based on Ribosomal ITS1 and ITS2 SequencesMycologia, 1996
- ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rustsMolecular Ecology, 1993
- Growth of Fusarium graminearum Schwabe group 1 on media amended with atrazine, chlorsulfuron or glyphosate in relation to temperature and osmotic potentialSoil Biology and Biochemistry, 1990
- PREDICTION OF THE PERSISTENCE OF THE TRIAZINE HERBICIDES ATRAZINE, CYANAZINE, AND METRIBUZIN IN REGINA HEAVY CLAYCanadian Journal of Soil Science, 1989
- The Allium test as a standard in environmental monitoringHereditas, 1985