Canine distemper virus induces downregulation of GABAA,GABAB, and GAT1 expression in brain tissue of dogs
- 6 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 165 (6), 1321-1331
- https://doi.org/10.1007/s00705-020-04617-3
Abstract
The aim of the study was to determine the expression profiles of GABAA, GABAB, and GAT1 using RT-PCR and the immunoreactivity of GAT1 via immunohistochemical and immunofluorescence assays in CDV-infected brain tissue of dogs. For this purpose, dogs with CDV and dogs without CDV were selected. The mRNA transcript levels of GABAA, GABAB, and GAT1 were significantly downregulated in brain tissue in the CDV-infected group as compared with that in non-CDV-infected brain tissue in the control group (p < 0.01, p < 0.001). In addition, the immunoreactivity of GAT1 in CDV-infected brain tissue was significantly lower than in the uninfected group (p < 0.05). We conclude that one of the main causes of myoclonus in CDV infections may be the blockage of postsynaptic inhibition in neurons or a lack of metabolism of GABA. In addition, a GABA neurotransmission imbalance could play a role in demyelination in CDV infections. Graphic abstractThis publication has 72 references indexed in Scilit:
- Nectin4 Is an Epithelial Cell Receptor for Canine Distemper Virus and Involved in NeurovirulenceJournal of Virology, 2012
- The altered expression of α1 and β3 subunits of the gamma-aminobutyric acid A receptor is related to the hepatitis C virus infectionEuropean Journal of Clinical Microbiology & Infectious Diseases, 2011
- Increased p75 neurotrophin receptor expression in the canine distemper virus model of multiple sclerosis identifies aldynoglial schwann cells that emerge in response to axonal damageGlia, 2011
- Canine Distemper Outbreak in Rhesus Monkeys, ChinaEmerging Infectious Diseases, 2011
- Beyond classical benzodiazepines: novel therapeutic potential of GABAA receptor subtypesNature Reviews Drug Discovery, 2011
- Canine Distemper Virus Selectively Inhibits Apoptosis Progression in Infected Immune CellsJournal of Virology, 2009
- Pathogenesis and immunopathology of systemic and nervous canine distemperVeterinary Immunology and Immunopathology, 2009
- International Union of Pharmacology. LXX. Subtypes of γ-Aminobutyric AcidAReceptors: Classification on the Basis of Subunit Composition, Pharmacology, and Function. UpdatePharmacological Reviews, 2008
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- GABAB Receptors: A New Paradigm in G Protein SignalingMolecular and Cellular Neuroscience, 2000