Combined QTL mapping and association study reveals candidate genes for leaf number and flowering time in maize
- 11 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 134 (10), 3459-3472
- https://doi.org/10.1007/s00122-021-03907-x
Abstract
Key message Twelve QTL for flowering and leaf number were detected. The ZmWRKY14Hap4 could increase leaf number, flowering time and biomass yield which are promising for silage maize breeding. Abstract Silage maize, one of the most important feedstock for ruminants, is widely grown from temperate regions to the tropics. Flowering time and leaf number are two significantly correlated traits and important for the quality, adaptation and biomass yield of silage maize. In this study, a recombinant inbred line population consisting of 215 individuals and an association panel of 369 inbred lines were analysed in field conditions in three locations for 2 consecutive years, and five, four and three quantitative trait loci for the total leaf number, days to anthesis (DTA) and silking (DTS) were detected, which could explain 48.55, 35.37 and 34.22% of total phenotypic variation, respectively. Association analysis of qLN10 on chromosome 10 found that ZmWRKY14 was the candidate gene for leaf number, whose expression level was negatively correlated with the leaf number. There are five haplotypes for ZmWRKY14, and haplotype 4 could significantly increase flowering time, leaf number and biomass yield, but has no obvious influence on ear weight. The optimal allelic combination of ZmWRKY14 and ZCN8 could further increase leaf number and biomass yield. The results will provide important genetic information for silage maize breeding.Funding Information
- Chinese Academy of Sciences (XDA24010106)
- the National Key Research and Development Plan of China (2016YFD0100605)
This publication has 63 references indexed in Scilit:
- The advantages and limitations of trait analysis with GWAS: a reviewPlant Methods, 2013
- ZmCCT and the genetic basis of day-length adaptation underlying the postdomestication spread of maizeProceedings of the National Academy of Sciences, 2012
- ZCN8 encodes a potential orthologue of Arabidopsis FT florigen that integrates both endogenous and photoperiod flowering signals in maizeJournal of Experimental Botany, 2011
- TheFT-LikeZCN8Gene Functions as a Floral Activator and Is Involved in Photoperiod Sensitivity in MaizePlant Cell, 2011
- Joint linkage–linkage disequilibrium mapping is a powerful approach to detecting quantitative trait loci underlying drought tolerance in maizeProceedings of the National Academy of Sciences of the United States of America, 2010
- Mixed linear model approach adapted for genome-wide association studiesNature Genetics, 2010
- Association Mapping: Critical Considerations Shift from Genotyping to Experimental DesignPlant Cell, 2009
- Fast model-based estimation of ancestry in unrelated individualsGenome Research, 2009
- Conserved noncoding genomic sequences associated with a flowering-time quantitative trait locus in maizeProceedings of the National Academy of Sciences, 2007
- A unified mixed-model method for association mapping that accounts for multiple levels of relatednessNature Genetics, 2005