Sevoflurane Postconditioning Reduces Hypoxia-Reoxygenation Injury in H9C2 Embryonic Rat Cardiomyocytes and Targets the STRADA Gene by Upregulating microRNA-107
- 25 April 2020
- journal article
- research article
- Published by International Scientific Information, Inc. in Medical Science Monitor
- Vol. 26, e920849-e920849-11
- https://doi.org/10.12659/MSM.920849
Abstract
Background: Sevoflurane as a widely used inhalational general anesthetic that also has a cardioprotective role in hypoxia-reoxygenation (H/R) injury. This study aimed to investigate the effects of microRNA-107 (miR-107) on sevoflurane postconditioning (SpostC) in H9C2 embryonic rat cardiomyocytes and to use bioinformatics analysis to identify the molecular basis of cardioprotection from sevoflurane in human cardiac tissue. Material/Methods: The STRADA gene was identified from the Gene Expression Omnibus (GEO) database. H9C2 embryonic rat cardiomyocytes were cultured with sevoflurane. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to measure the mRNA expression and protein expression of STRADA and miR-107 in H9C2 cells. TargetScanHuman version 7.2 was used to identify the target gene of miR-107 and to predict the STRADA 3'-UTR binding site of miR-107. The dual-luciferase reporter assay measured the relative luciferase activity. The cell proliferation rate and cell apoptosis were measured using the MU assay and flow cytometry, respectively. Results: H/R injury in H9C2 cells following SpostC resulted in increased expression of miR-107 and reduced expression of STRADA. Specific binding of miR-107 was identified to STRADA 3'-UTR. Upregulation of the miR-107 in SpostC H/R injured H9C2 cells promoted cell proliferation, reduced cell apoptosis, and downregulating the protein expression of caspase-3. STRADA overexpression reduced the effects of a miR-107 mimic on SpostC. Conclusions: SpostC reduced H/R injury in H9C2 embryonic rat cardiomyocytes by targeting the STRADA gene and by up- regulating the expression of microRNA-107.Keywords
This publication has 43 references indexed in Scilit:
- Mitochondrial Permeability Transition and Cell Death: The Role of Cyclophilin DFrontiers in Physiology, 2013
- The Na+/Ca2+ exchanger in cardiac ischemia/reperfusion injuryMedical Science Monitor, 2012
- STRADα deficiency results in aberrant mTORC1 signaling during corticogenesis in humans and miceJCI Insight, 2010
- ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour SuppressorPLoS Biology, 2009
- STRADα Regulates LKB1 Localization by Blocking Access to Importin-α, and by Association with Crm1 and Exportin-7Molecular Biology of the Cell, 2008
- Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5Brain, 2007
- Expression of an active LKB1 complex in cardiac myocytes results in decreased protein synthesis associated with phenylephrine-induced hypertrophyAmerican Journal of Physiology-Heart and Circulatory Physiology, 2007
- Preconditioning, postconditioning and their application to clinical cardiologyCardiovascular Research, 2006
- Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRADThe EMBO Journal, 2003
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001