Optical Manipulation of F-Actin with Photoswitchable Small Molecules
- 11 May 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 142 (20), 9240-9249
- https://doi.org/10.1021/jacs.9b12898
Abstract
Cell-permeable photoswitchable small molecules, termed optojasps, are introduced to optically control the dynamics of the actin cytoskeleton and cellular functions that depend on it. These light-dependent effectors were designed from the F-actin-stabilizing marine depsipeptide jasplakinolide by functionalizing them with azobenzene photoswitches. As demonstrated, optojasps can be employed to control cell viability, cell motility, and cytoskeletal signaling with the high spatial and temporal resolution that light affords. Optojasps can be expected to find applications in diverse areas of cell biological research. They may also provide a template for photopharmacology targeting the ubiquitous actin cytoskeleton with precision control in the micrometer range.Funding Information
- Deutscher Akademischer Austauschdienst
- Th?ringer Ministerium f?r Wirtschaft, Wissenschaft und Digitale Gesellschaft (43-5572-321-12040-12)
- Deutsche Forschungsgemeinschaft (EXS 2051, SFB 1032, SFB 152)
- National Institutes of Health (R01 GM126228)
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