Molecular analysis of the blood meals and bacterial communities of bed bugs (Cimex lectularius L.) to assess interactions with alternative hosts
- 9 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 120 (4), 1209-1217
- https://doi.org/10.1007/s00436-021-07079-x
Abstract
Common bed bugs (Cimex lectularius L.) are hematophagous pests present in urban environments across the globe. It is widely established that they have a strong host preference for humans. However, there are records of C. lectularius feeding upon a range of mammalian and avian hosts, including rodents, in the field. There is little information available about how frequently common bed bugs feed on alternative hosts in residential settings, but understanding this phenomenon has implications for both management of infestations and public health. Here, we examined cohorts of C. lectularius collected from 13 different dwellings in the state of New Jersey, USA, that were known to be simultaneously infested with house mice (Mus musculus domesticus). Host-specific quantitative polymerase chain reaction (qPCR) was used to determine if blood meals were taken from mice, while 16S rRNA gene amplicon sequencing was used to screen the bed bugs for the presence of zoonotic bacterial pathogens. We found no evidence that any of the bed bugs we collected fed on mice. Furthermore, the insects harbored depauperate bacterial communities that did not include known human pathogens. However, host-specific qPCR detected feline DNA in a pool of bed bugs from one dwelling, suggesting that interaction with domestic pets should be further investigated. Although sampling in this study was limited, the approach described herein will be useful for additional studies of the interactions between bed bugs and alternative blood meal hosts.Keywords
This publication has 47 references indexed in Scilit:
- phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census DataPLOS ONE, 2013
- A 454 Survey Reveals the Community Composition and Core Microbiome of the Common Bed Bug (Cimex lectularius) across an Urban LandscapePLOS ONE, 2013
- The SILVA ribosomal RNA gene database project: improved data processing and web-based toolsNucleic Acids Research, 2012
- Establishment of a molecular tool for blood meal identification in MalaysiaAsian Pacific Journal of Tropical Biomedicine, 2012
- Mitochondrial DNA and morphology show independent evolutionary histories of bedbug Cimex lectularius (Heteroptera: Cimicidae) on bats and humansZeitschrift Fur Parasitenkunde-Parasitology Research, 2012
- Measuring dementia carers' unmet need for services - an exploratory mixed method studyBMC Health Services Research, 2010
- Ectoparasites from Feral Pigeons Affecting HumansDermatology, 2009
- A New Method for Forensic DNA Analysis of the Blood Meal in Chagas Disease Vectors Demonstrated Using Triatoma infestans from Chuquisaca, BoliviaPLOS ONE, 2008
- Susceptibility of the Bed Bug Cimex lectularius L. (Heteroptera: Cimicidae) Collected in Poultry Production Facilities to Selected InsecticidesJournal of Agricultural and Urban Entomology, 2008
- The occurrence of the tropical bedbug (Cimex hemipterus, fabricius) in poultry barns in IsraelAvian Pathology, 1987