The effects of follicle-stimulating hormone receptor (FSHR) -29 and Ser680Asn polymorphisms in IVF/ICSI
- 1 June 2020
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hormone Molecular Biology and Clinical Investigation
- Vol. 41 (2)
- https://doi.org/10.1515/hmbci-2019-0058
Abstract
Background: The aim of this study was to analyze two different polymorphisms, Ser680Asn and -29 (G>A) promoter polymorphism, of the follicle-stimulating hormone receptor (FSHR) gene, individually but also in combination, in a sample of Greek women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Materials and methods: One hundred and forty-one women undergoing IVF or ICSI and 94 controls were genotyped by real-time polymerase chain reaction (RT-PCR) for the two FSHR polymorphisms. The association of the alleles with the clinical, biochemical and other parameters concerning the controlled ovarian stimulation (COS) protocol and outcome was investigated, as well as the pregnancy rate. Results: The study of each polymorphism individually revealed a positive correlation of the SerSer genotype (Ser680Asn polymorphism) with higher luteinizing hormone (LH) levels on the third day of the menstrual cycle. On the other hand, the A allele for the -29 (G>A) promoter polymorphism correlated with the increased number and quality of cumulus-oocyte complexes (COCs). No differences were detected when the different genotypes of the two polymorphisms were combined - the population study was grouped according to the number of polymorphic alleles they carried (0-4 alleles). Women who presented all polymorphic alleles, As-nAsn/AA, exhibited the lowest LH levels (2.62 +/- 0.68 mIU/L), but were rarely detected (n = 2, 1.4% of the studied population). Conclusions: The data from this study reflect that the investigation of the combination of polymorphisms, such as FSHR -29 and Ser680Asn, could offer a valuable tool in order to evaluate and anticipate the outcome of the ovulation induction protocols, especially in the group of patients with failed attempts.Keywords
This publication has 27 references indexed in Scilit:
- A common Asn680Ser polymorphism in the follicle-stimulating hormone receptor gene is not associated with ovarian response to gonadotropin stimulation in patients undergoing in vitro fertilizationFertility and Sterility, 2013
- LH receptor gene expression in cumulus cells in women entering an ART programJournal of Assisted Reproduction and Genetics, 2012
- In Vitro Fertilization, the Nobel Prize, and Human Embryonic Stem CellsCell Stem Cell, 2011
- Current Concepts of Follicle-Stimulating Hormone Receptor Gene Regulation1Biology of Reproduction, 2011
- A distribution of two SNPs in exon 10 of the FSHR gene among the women with a diminished ovarian reserve in UkraineJournal of Assisted Reproduction and Genetics, 2008
- FSH receptor gene polymorphisms have a role for different ovarian response to stimulation in patients entering IVF/ICSI-ET programsJournal of Assisted Reproduction and Genetics, 2006
- Genetic complexity of FSH receptor functionTrends in Endocrinology & Metabolism, 2005
- Single-nucleotide polymorphisms in the promoter region influence the expression of the human follicle-stimulating hormone receptorFertility and Sterility, 2005
- Significance of a common single nucleotide polymorphism in exon 10 of the follicle-stimulating hormone (FSH) receptor gene for the ovarian response to FSH: a pharmacogenetic approach to controlled ovarian hyperstimulationPharmacogenetics and Genomics, 2005
- Human controlled ovarian hyperstimulation outcome is a polygenic traitPharmacogenetics, 2004