Hepatitis B Virus DNA Integration: In Vitro Models for Investigating Viral Pathogenesis and Persistence
Open Access
- 25 January 2021
- Vol. 13 (2), 180
- https://doi.org/10.3390/v13020180
Abstract
Hepatitis B virus (HBV) is a globally-distributed pathogen and is a major cause of liver disease. HBV (or closely-related animal hepadnaviruses) can integrate into the host genome, but (unlike retroviruses) this integrated form is replication-defective. The specific role(s) of the integrated HBV DNA has been a long-standing topic of debate. Novel in vitro models of HBV infection combined with sensitive molecular assays now enable researchers to investigate this under-characterised phenomenon with greater ease and precision. This review covers the contributions these systems have made to understanding how HBV DNA integration induces liver cancer and facilitates viral persistence. We summarise the current findings into a working model of chronic HBV infection and discuss the clinical implications of this hypothetical framework on the upcoming therapeutic strategies used to curb HBV-associated pathogenesis.Keywords
Funding Information
- Deutsches Zentrum für Infektionsforschung (Project 5.704)
This publication has 152 references indexed in Scilit:
- Clonal Expansion of Normal-Appearing Human Hepatocytes during Chronic Hepatitis B Virus InfectionJournal of Virology, 2010
- Nuclear Entry of Hepatitis B Virus Capsids Involves Disintegration to Protein Dimers followed by Nuclear Reassociation to CapsidsPLoS Pathogens, 2009
- Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycansHepatology, 2007
- Base Pairing between cis -Acting Sequences Contributes to Template Switching during Plus-Strand DNA Synthesis in Human Hepatitis B VirusJournal of Virology, 2007
- The Sequence of the RNA Primer and the DNA Template Influence the Initiation of Plus-strand DNA Synthesis in Hepatitis B VirusJournal of Molecular Biology, 2007
- Complementarity between epsilon and phi sequences in pregenomic RNA influences hepatitis B virus replication efficiencyVirology, 2007
- Treatment of acetaminophen-induced acute liver failure in the mouse with conditionally immortalized human hepatocytesJournal of Hepatology, 2005
- Production of hepatitis B virus by a differentiated human hepatoma cell line after transfection with cloned circular HBV DNACell, 1986
- Integration of HBV-DNA may not be a prerequisite for the maintenance of the state of malignant transformationJournal of Hepatology, 1986
- Integration of Hepatitis B Virus DNA into the Genome of Liver Cells in Chronic Liver Disease and Hepatocellular CarcinomaNew England Journal of Medicine, 1981