Comparative biotransformation of luteolin and apigenin from the flower extract and the stem‐and‐leaf extract of Dendranthema morifolium Ramat. Tzvel. in rats
- 4 February 2021
- journal article
- research article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 101 (12), 4934-4945
- https://doi.org/10.1002/jsfa.11137
Abstract
BACKGROUND The flower of Dendranthema morifolium Ramat Tzvel has been widely used as a nutritional health supplement worldwide. However, most of the studies have focused on the flower and the rest of the plant was neglected. Our hypothesis is that similar flavonoids may be present at different parts of D. morifolium, and the flavonoids may undergo a similar biotransformation pathway within the body. To investigate this hypothesis, an in vivo pharmacokinetic experimental model was developed to explore the comparative biotransformation of luteolin and apigenin after administration of D. morifolium extracts (10 g kg−1, p.o.) in freely moving rats. Because luteolin and apigenin mainly underwent phase II metabolism, the metabolic enzymes of β‐glucuronidase/sulfatase or β‐glucuronidase were used to hydrolyze the plasma sample, depending on the biotransformation pathway involved. RESULTS The results revealed that luteolin and apigenin mainly went through glucuronide and sulfate conjugations, respectively, in both the extract of flowers and the stem‐and‐leaf group. In addition, the area under the concentration curve (AUClast) of luteolin glucuronides and sulfates in the group administered the stem‐and‐leaf extract was approximately 4.6 times higher than that of the flower extract group. The dominant products of biotransformation for apigenin were sulfates. CONCLUSION These findings support our hypothesis that not only the flower parts of D. morifolium, but also the stem‐and‐leaf parts contain rich flavones, including glycosides and aglycone, and they undergo similar biotransformation pathways. © 2021 Society of Chemical IndustryKeywords
Funding Information
- Ministry of Science and Technology, Taiwan (MOST 107‐2113‐M‐010‐005, MOST 109‐2113‐M‐010‐007)
- Ministry of Science and Technology, Taiwan
This publication has 45 references indexed in Scilit:
- Sustainable Medicines and Global Health CarePlanta Medica, 2011
- Relative Contribution of Small and Large Intestine to Deglycosylation and Absorption of Flavonoids from Chrysanthemun morifolium ExtractJournal of Agricultural and Food Chemistry, 2010
- Identification of the phenolic components of chrysanthemum flower (Chrysanthemum morifolium Ramat)Food Chemistry, 2009
- Cytotoxic activity of flavonoids from the flowers of Chrysanthemum morifolium on human colon cancer Colon205 cellsJournal of Asian Natural Products Research, 2009
- Herbal Products and GABA ReceptorsPublished by Elsevier ,2009
- Absorption and Excretion of Luteolin and Apigenin in Rats after Oral Administration of Chrysanthemum morifolium ExtractJournal of Agricultural and Food Chemistry, 2006
- Influence of Biotransformation of Luteolin, Luteolin 7-O-Glucoside, 3′,4′-Dihydroxyflavone and Apigenin by Cultured Rat Hepatocytes on Antioxidative Capacity and Inhibition of EGF Receptor Tyrosine Kinase ActivityPlanta Medica, 2006
- Evaluation of the antioxidant activity of flavonoids by “ferric reducing antioxidant power” assay and cyclic voltammetryBiochimica et Biophysica Acta (BBA) - General Subjects, 2004
- METABOLISM OF APIGENIN BY RAT LIVER PHASE I AND PHASE II ENZYMES AND BY ISOLATED PERFUSED RAT LIVERDrug Metabolism and Disposition, 2004
- Apigenin, a Component ofMatricaria recutitaFlowers, is a Central Benzodiazepine Receptors-Ligand with Anxiolytic EffectsPlanta Medica, 1995