Multi-functional MnO2-doped Fe3O4 nanoparticles as an artificial enzyme for the colorimetric detection of bacteria
- 1 May 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 412 (13), 3135-3140
- https://doi.org/10.1007/s00216-020-02563-2
Abstract
The authors describe a rapid enzyme-free colorimetric assay for food- and water-borne pathogens by using the multi-functional MnO2-doped magnetic ferrite nanoparticles (MCDs-MnO2 NPs). These nanoparticles not only can recognize, absorb, and separate the analyte from the matrix efficiently but also can directly catalyze the oxidation of TMB into a blue colored product without H2O2. In the presence of target, the nanoparticles preferentially bind to the target surface and the interaction between nanoparticles and TMB can be blocked, providing turn-off sensing of bacteria. By combining the superior capture efficiency of magnetic beads with the high catalytic activity of the enzyme mimic, the turn-off colorimetric assay can detect the gram-positive bacterium and the gram-negative bacterium by the naked eye at a low detection limit (10(2) cfu mL(-1)) with a wide line arrange from 10 to 10(6) cfu mL(-1). With the advantage of simplicity, sensitivity, and specificity, this proposed approach showed promise in rapid instrumental and on-site visual detection of bacteria for determination of water contamination.Keywords
Funding Information
- National Natural Science Foundation of China (81872668)
- Norman Bethune Health Science Center of Jilin University (2018A05)
- Health Commission of Jilin Province (2018Q033)
- Education Department of Jilin Province (JJKH20180240KJ)
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