Current Technical Approaches for the Early Detection of Foodborne Pathogens: Challenges and Opportunities
Open Access
- 29 September 2017
- journal article
- review article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 18 (10), 2078
- https://doi.org/10.3390/ijms18102078
Abstract
The development of novel and high-tech solutions for rapid, accurate, and non-laborious microbial detection methods is imperative to improve the global food supply. Such solutions have begun to address the need for microbial detection that is faster and more sensitive than existing methodologies (e.g., classic culture enrichment methods). Multiple reviews report the technical functions and structures of conventional microbial detection tools. These tools, used to detect pathogens in food and food homogenates, were designed via qualitative analysis methods. The inherent disadvantage of these analytical methods is the necessity for specimen preparation, which is a time-consuming process. While some literature describes the challenges and opportunities to overcome the technical issues related to food industry legal guidelines, there is a lack of reviews of the current trials to overcome technological limitations related to sample preparation and microbial detection via nano and micro technologies. In this review, we primarily explore current analytical technologies, including metallic and magnetic nanomaterials, optics, electrochemistry, and spectroscopy. These techniques rely on the early detection of pathogens via enhanced analytical sensitivity and specificity. In order to introduce the potential combination and comparative analysis of various advanced methods, we also reference a novel sample preparation protocol that uses microbial concentration and recovery technologies. This technology has the potential to expedite the pre-enrichment step that precedes the detection process.Keywords
This publication has 69 references indexed in Scilit:
- Multifunctional nanoparticles for rapid bacterial capture, detection, and decontaminationRSC Advances, 2013
- Enhancing Nanoparticle-Based Visible Detection by Controlling the Extent of AggregationScientific Reports, 2012
- Noble Metal Nanoparticles for Biosensing ApplicationsSensors, 2012
- Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial PathogensBiosensors, 2012
- Colorimetric Bacteria Sensing Using a Supramolecular Enzyme–Nanoparticle BiosensorJournal of the American Chemical Society, 2011
- Single-walled carbon nanotube chemoresistive label-free immunosensor for salivary stress biomarkersThe Analyst, 2010
- Ultrasensitive Detection of Bacteria Using Core–Shell Nanoparticles and an NMR‐Filter SystemAngewandte Chemie-International Edition, 2009
- Nanowire sensors for multiplexed detection of biomoleculesCurrent Opinion in Chemical Biology, 2008
- Improving the photostability of bright monomeric orange and red fluorescent proteinsNature Methods, 2008
- Rapid transport of large polymeric nanoparticles in fresh undiluted human mucusProceedings of the National Academy of Sciences, 2007