Period of Boar Ejaculate Collection Contributes to the Yearly Intra-Male Variability of Seminal Plasma Cytokines
Open Access
- 19 May 2020
- Vol. 9 (5), 105
- https://doi.org/10.3390/biology9050105
Abstract
The concentrations of cytokines in seminal plasma (SP) fluctuate over time in healthy males, weakening their practical usefulness as diagnostic tools. This study evaluated the relevance of intra-male variability in SP cytokines and to what extent the period of the year when ejaculate is collected contributes to such variability. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, and TNFα) were measured using the Luminex xMAP® technology for 180 SP samples of ejaculate collected over a year from nine healthy and fertile boars. The SP samples were grouped into two annual periods according to decreasing or increasing daylight and ambient temperature. Intra-male variability was higher than inter-male variability for all cytokines. All SP cytokines showed concentration differences between the two periods of the year, showing the highest concentration during the increasing daylength/temperature period, irrespective of the male. Similarly, some cytokines showed differences between daylength/temperature periods when focusing on their total amount in the ejaculate. No strong relationship (explaining more than 50% of the total variance) was found between annual fluctuations in SP-cytokine levels and semen parameters. In conclusion, the period of the year during which ejaculates were collected helps explain the intra-male variability of SP-cytokine levels in breeding boars.Keywords
Funding Information
- European Regional Development Fund (AGL2015-69738-R)
This publication has 54 references indexed in Scilit:
- Seminal Fluid and Immune Adaptation for Pregnancy – Comparative Biology in Mammalian SpeciesReproduction in Domestic Animals, 2014
- Seminal fluid and reproduction: much more than previously thoughtJournal of Assisted Reproduction and Genetics, 2014
- Seminal Plasma: An Essential Attribute to SpermatozoaJournal of Andrology, 2012
- Seminal Plasma Proteins as Modulators of the Sperm Function and Their Application in Sperm BiotechnologiesReproduction in Domestic Animals, 2012
- Seasonal changes in antioxidant defence systems in seminal plasma and fluids of the boar reproductive tractReproductive Biology, 2011
- Quantification of factors affecting semen traits in artificial insemination boars from animal model analyses1Journal of Animal Science, 2009
- Natural Mediterranean photoperiod does not affect the main parameters of boar-semen quality analysisTheriogenology, 2005
- Management and sperm production of boars under differing environmental conditionsTheriogenology, 2004
- Semen quality of postpubertal boars during increasing and decreasing natural photoperiodsTheriogenology, 2004
- Seasonal effect on steroids in blood plasma and seminal plasma of boarsJournal of Steroid Biochemistry, 1983