Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon

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Abstract
An electrochemical immunoassay for the carcinoembryonic antigen is described. It is based on the use of Au NPs modified zeolitic imidazolate framework (ZIF-8) and ordered mesoporous carbon (OMC). Au NPs@ZIF-8 was synthesized by reduction of chloroauric acid. It serves as immobilization support nanocarrier to increase antibody loading due to its large surface area. OMC was dropped on a glassy carbon electrode to improve electrochemical signals due to enhanced electrical conductivity. Differential pulse voltammetry was carried out to record electrochemical responses (best measured at 0.26 V vs. Ag/AgCl). The immunosensor demonstrated excellent electrochemical performance with a linear determination range of 5 pg mL−1 to 400 ng mL−1 and a determination limit of 1.3 pg mL−1 (S/N = 3). The sensor also exhibited high selectivity, good stability, and acceptable reproducibility. Scheme 1 Schematic representation of fabrication of the immunosensor for CEA determination
Funding Information
  • National Natural Science Foundation of China (81773480, 81573200, 81673229)
  • National Outstanding Youth Science Fund Project of National Natural Science Foundation of China (81703269)
  • major weak subjects in Pudong New Area (PWZbr2017-02)
  • National Natural Science Fund of China (81973097)
  • Shanghai Pudong Hospital Talent Project (PX201604)
  • Natural Science Foundation of Heilongjiang Province (QC2018120)
  • University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2017173)
  • fundamental research funds for the universities of heilongjiang province (2017-KYYWF-0648)
  • the Administration of Science & Technology Foundation of Mudanjiang (No. Z2016s0072)

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