Study on the effect of sodium-nanoparticle in rice root development

Abstract

Micro-nanoparticles can enter the root tissue of plant cells along with the multiple lanes, and then accumulate in the tissue. But the plant physiological effect is still less studied. In this study, rice seedlings at germination stage were treated with 100 µM NaBiF₄ and BiF₃. We found that exogenous application of NaBiF₄ treatment inhibited the elongation of rice roots and promoted the generation of adventitious roots, but treated BiF₃ did not mediate obvious phenotype. Further analysis of the peroxidase activity in related tissues showed that NaBiF₄ induced the activity of SOD and CAT decreased, and POD increased, while BiF₃ only induced the activity of SOD to reduced, but the activity of CAT and POD were no changed. Further analysis of the sodium element and potassium element concentration in tissues showed that only the NaBiF₄ treatment reduced content of potassium, but not sodium. Finally, stress-related genes OsMT1, OsMT2, OsOVP1, OsNIP2;1, and OsMT2b were analyzed by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). These results showed that NaBiF₄ induced the expression of OsMT2, OsOVP1, OsNIP2;1 decreased, and OsMT2b increased. However, BiF₃ only induced expression of OsMT1 increased. These results provide a physiological basis for further analysis of the effects of sodium salt-nanoparticles in crop plants.