Association between Breastfeeding and DNA Methylation over the Life Course: Findings from the Avon Longitudinal Study of Parents and Children (ALSPAC)
Open Access
- 29 October 2020
- Vol. 12 (11), 3309
- https://doi.org/10.3390/nu12113309
Abstract
Background: Breastfeeding is associated with short and long-term health benefits. Long-term effects might be mediated by epigenetic mechanisms, yet the literature on this topic is scarce. We performed the first epigenome-wide association study of infant feeding, comparing breastfed vs non-breastfed children. We measured DNA methylation in children from peripheral blood collected in childhood (age 7 years, N = 640) and adolescence (age 15–17 years, N = 709) within the Accessible Resource for Integrated Epigenomic Studies (ARIES) project, part of the larger Avon Longitudinal Study of Parents and Children (ALSPAC) cohort. Cord blood methylation (N = 702) was used as a negative control for potential pre-natal residual confounding. Results: Two differentially-methylated sites presented directionally-consistent associations with breastfeeding at ages 7 and 15–17 years, but not at birth. Twelve differentially-methylated regions in relation to breastfeeding were identified, and for three of them there was evidence of directional concordance between ages 7 and 15–17 years, but not between birth and age 7 years. Conclusions: Our findings indicate that DNA methylation in childhood and adolescence may be predicted by breastfeeding, but further studies with sufficiently large samples for replication are required to identify robust associations.Keywords
Funding Information
- Medical Research Council (MC_UU_00011/1, MC_UU_00011/5)
- Wellcome Trust (102215/2/13/2)
This publication has 63 references indexed in Scilit:
- Comb-p: software for combining, analyzing, grouping and correcting spatially correlatedP-valuesBioinformatics, 2012
- DNA methylation arrays as surrogate measures of cell mixture distributionBMC Bioinformatics, 2012
- Cohort Profile: The Avon Longitudinal Study of Parents and Children: ALSPAC mothers cohortInternational Journal of Epidemiology, 2012
- Cohort Profile: The ‘Children of the 90s’—the index offspring of the Avon Longitudinal Study of Parents and ChildrenInternational Journal of Epidemiology, 2012
- Bump hunting to identify differentially methylated regions in epigenetic epidemiology studiesInternational Journal of Epidemiology, 2012
- Commentary: The seven plagues of epigenetic epidemiologyInternational Journal of Epidemiology, 2012
- Epigenome-wide association studies for common human diseasesNature Reviews Genetics, 2011
- What are the causal effects of breastfeeding on IQ, obesity and blood pressure? Evidence from comparing high-income with middle-income cohortsInternational Journal of Epidemiology, 2011
- Comparison of Beta-value and M-value methods for quantifying methylation levels by microarray analysisBMC Bioinformatics, 2010
- Principal components analysis corrects for stratification in genome-wide association studiesNature Genetics, 2006