Low-Level Laser Irradiation Promotes Proliferation and Differentiation of Human Osteoblastsin Vitro
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- 1 April 2005
- journal article
- research article
- Published by Mary Ann Liebert Inc in Photomedicine and Laser Surgery
- Vol. 23 (2), 161-166
- https://doi.org/10.1089/pho.2005.23.161
Abstract
Objectives: The aim of the present study was to investigate the effect of low-level laser irradiation on proliferation and differentiation of a human osteoblast cell line. Background data: It was previously found that lowlevel laser therapy (LLLT) enhances bone repair in experimental models. Materials and methods: Cultured osteoblast cells were irradiated using He-Ne laser irradiation (632 nm; 10 mW power output). On the second and third day after seeding the osteoblasts were exposed to laser irradiation. The effect of irradiation on osteoblast proliferation was quantified by cell count and colorimetric MTT (dimethylthiazol tetrazolium bromide) assay 24 and 48 h after second irradiation. Results: Asignificant 31–58% increase in cell survival (MTT assay) and higher cell count in the once-irradiated as compared to nonirradiated cells was monitored. Differentiation and maturation of the cells was followed by osteogenic markers: alkaline phosphatase (ALP), osteopontin (OP), and bone sialoprotein (BSP). A two-fold enhancement of ALP activity and expression of OP and BSP was much higher in the irradiated cells as compared to non-irradiated osteoblasts. Conclusion: We conclude that LLLT promotes proliferation and maturation of human osteoblasts in vitro. These results may have clinical implications.Keywords
This publication has 32 references indexed in Scilit:
- Attenuation of infarct size in rats and dogs after myocardial infarction by low‐energy laser irradiationLasers in Surgery and Medicine, 2001
- Low-energy laser irradiation affects satellite cell proliferation and differentiation in vitroBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1999
- The Mature Osteoblast Phenotype Is Characterized by Extensive PlasticityExperimental Cell Research, 1997
- EFFECTS OF LOW LEVEL LASER IRRADIATION (LLLI) WITH 904nm PULSED DIODE LASER ON OSTEOBLASTS: A CONTROLLED TRIAL WITH THE RAT OSTEOBLAST MODELLASER THERAPY, 1996
- Effect of Low-Energy Laser (He-Ne) Irradiation on the Process of Bone Repair in the Rat TibiaBone, 1995
- Effect of low‐intensity argon laser irradiation on mitochondrial respirationLasers in Surgery and Medicine, 1994
- Pulsed magnetic fields improve osteoblast activity during the repair of an experimental osseous defectJournal of Orthopaedic Research, 1993
- Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiationThe Anatomical Record, 1993
- The BMP proteins in bone formation and repairTrends in Genetics, 1992
- Photobiology of Low-power Laser EffectsHealth Physics, 1989