Characterization and Validation of an Isotope-Dilution LC–MS/MS Method for Quantification of Total Desmosine and Isodesmosine in Plasma and Serum
- 13 August 2013
- journal article
- research article
- Published by Informa UK Limited in Bioanalysis
- Vol. 5 (16), 1991-2001
- https://doi.org/10.4155/bio.13.164
Abstract
Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. RESULTS/METHODOLOGY: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.Keywords
Funding Information
- Translational Medicine Research Collaboration (Grants AU124, DU106 and GU90)
This publication has 22 references indexed in Scilit:
- Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder using ultra performance liquid chromatography–ion mobility-mass spectrometryJournal of Chromatography B, 2011
- Quantitation of desmosine and isodesmosine in urine, plasma, and sputum by LC–MS/MS as biomarkers for elastin degradationJournal of Chromatography B, 2011
- The effect of tiotropium therapy on markers of elastin degradation in COPDRespiratory Research, 2009
- High-Sensitivity NanoLC−MS/MS Analysis of Urinary Desmosine and IsodesmosineAnalytical Chemistry, 2009
- Desmosine as a biomarker of elastin degradation in COPD: current status and future directionsEuropean Respiratory Journal, 2008
- COPD as a Systemic DiseaseCOPD: Journal of Chronic Obstructive Pulmonary Disease, 2008
- Association between markers of emphysema and more severe chronic obstructive pulmonary diseaseThorax, 2006
- THE KINETICS OF ELASTOLYSIS: Elastin Catabolism During Experimentally Induced FibrosisExperimental Lung Research, 1999
- Identification of an Elastin Cross-linking Domain That Joins Three Peptide ChainsJournal of Biological Chemistry, 1995
- INTERPRETATION OF URINE RESULTS USED TO ASSESS CHEMICAL EXPOSURE WITH EMPHASIS ON CREATININE ADJUSTMENTS: A REVIEWAihaj Journal, 1993