Radioimmunoassay of Plasma Corticosterone
- 1 February 1974
- journal article
- research article
- Published by Informa UK Limited in Analytical Letters
- Vol. 7 (2), 109-118
- https://doi.org/10.1080/00032717408058744
Abstract
This report describes a radioimmunoassay method for the measurement of unconjugated corticosterone (B) in plasma. Utilizing an anti-Cortisol antiserum cross reacting with B combined with a one step celite microcolumn chromatographic system, we have measured this latter steroid accurately in small aliquots of plasma from both male and female subjects. The plasma levels of B obtained by this method agreed well with levels observed in adult men and women, using other techniques. During a menstrual cycle studied, wide fluctuations were observed with levels ranging from 1, 3 to 14, 4 ng/ml. Adrenal suppression decreased the levels of B below 1 ng/ml. The mineralocorticoid B is predominantly ACTH -dependent.Keywords
This publication has 8 references indexed in Scilit:
- Plasma 11-deoxycortisol radioimmunoassay for metyrapone testsSteroids, 1972
- Radioimmunoassay of Plasma CortisolAnalytical Letters, 1972
- Radioimmunoassay of Plasma 17-HydroxypregnenoloneAnalytical Letters, 1972
- Radioimmunoassay of Plasma ProgesteroneJournal of Clinical Endocrinology & Metabolism, 1971
- Solid-Phase Radioimmunoassay of Estradiol-17βJournal of Clinical Endocrinology & Metabolism, 1969
- DOUBLE ISOTOPE ASSAY OF ALDOSTERONE, CORTICOSTERONE AND CORTISOL IN HUMAN PERIPHERAL PLASMAJournal of Endocrinology, 1968
- Some Studies of the Protein-Binding of Steroids and Their Application to the Routine Micro and Ultramicro Measurement of Various Steroids in Body Fluids by Competitive Protein-Binding Radioassay1Journal of Clinical Endocrinology & Metabolism, 1967
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJCI Insight, 1960