Abstract
When autotrophically growing cultures of Chlorella are treated with levulinic acid, delta-aminolevulinic acid is excreted into the medium, providing a direct demonstration of alpha-aminolevulinic acid production in a green plant. Evidence is presented which indicates that alpha-aminolevulinic acid formation may be the the rate-controlling step of chlorophyll synthesis in Chlorella, and that control of the rate of alpha-aminolevulinic acid synthesis may be exerted at the level of production and breakdown of an enzyme which catalyzes its formation.