Studies on Propagation of the Col SK Group of Viruses in Various Tissue Culture Media.

Abstract

L cells, HeLa cells and KB cells supported cytopathogenic growth of EMC and of Mengo virus; F virus grew only on L cells; and Col SK and MM viruses failed to grow in any of the 3 substrates. HeLa tissue cultures inoculated with EMC virus, in remote serial passage, were infectious for HeLa cells and for mice at an ID50 of about 105/1 cc of culture fluid and had a hemagglutinating titer of between 12 and 1:4. Data permitting the establishment of a growth curve of EMC tissue culture virus on HeLa cells are submitted.