Separation of Selected Flavonoids by use of RP?HPLC/NP?HPTLC Coupled Methods

Abstract

Phenolic compounds, such as phenolic acids, flavonoids, flavonoid glycosides, and resveratrol, were chromatographed on thin‐layers of silica in various eluent systems. Systems with the highest selectivity were chosen. Also, reversed‐phase HPLC systems were optimized for the separation of investigated compounds and most the selective system was applied for the separation of two phenolic extracts – Polygonum avicularis and Polygonum hydropiper. Partly separated fractions were evaporated and dissolved in methanol. Fractions with partly separated phenolic compounds were spotted onto the silica layer and developed in an appropriate eluent system. After drying, the plate was derivatised with the Naturstoff reagent and videoscanned. Several phenolic compounds were identified, according to their retention coefficients in both systems.