Cortisol Modification of HeLa 65 Alkaline Phosphatase
- 1 January 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 61 (2), 493-499
- https://doi.org/10.1111/j.1432-1033.1976.tb10044.x
Abstract
Alkaline phosphatase (EC 3.1.3.1) activity of HeLa cells is increased 5- to 20-fold during growth in medium with cortisol. The increase in enzyme activity is due to an enhanced catalytic efficiency rather than an increase in alkaline phosphatase protein in induced cells. The chemical composition of control and induced forms of alkaline phosphatase were investigated to determine the enzyme modification that may be responsible for the increased catalytic activity. HeLa alkaline phosphatase is a phosphoprotein and the induced form of the enzyme has approximately 1/2 of the phosphate residues associated with control enzyme. The decrease in phosphate residues of the enzyme apparently alters its catalytic activity. Other chemical components of purified alkaline phosphatase from control and induced cells are similar; these include sialic acid, hexosamine and sulfhydryl residues.This publication has 41 references indexed in Scilit:
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