Host Ranges of Listeria -Specific Bacteriophages from the Turkey Processing Plant Environment in the United States
- 1 November 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (21), 6623-6630
- https://doi.org/10.1128/aem.01282-08
Abstract
Even though at least 400 Listeria phages have been isolated from various sources, limited information is available on phages from the food processing plant environment. Phages in the processing plant environment may play critical roles in determining the Listeria population that becomes established in the plant. In this study, we pursued the isolation of Listeria -specific phages from environmental samples from four turkey processing plants in the United States. These environmental samples were also utilized to isolate Listeria spp. Twelve phages were isolated and classified into three groups in terms of their host range. Of these, nine (group 1) showed a wide host range, including multiple serotypes of Listeria monocytogenes , as well as other Listeria spp. ( L. innocua , L. welshimeri , L. seeligeri , and L. ivanovii ). The remaining phages mostly infected L. monocytogenes serotype 4b as well as L. innocua , L. ivanovii , and/or L. welshimeri . All but one of the strains of the serotype 4b complex (4b, 4d, 4e) from the processing plant environment could be readily infected by the wide-host-range phages isolated from the environment of the processing plants. However, many strains of other serotypes (1/2a [or 3a] and 1/2b [or 3b]), which represented the majority of L. monocytogenes strains isolated from the environmental samples, were resistant to infection by these phages. Experiments with two phage-resistant strains showed reduced phage adsorption onto the host cells. These findings suggest that phage resistance may be an important component of the ecology of L. monocytogenes in the turkey processing plants.Keywords
This publication has 24 references indexed in Scilit:
- Genomic Divisions/Lineages, Epidemic Clones, and Population StructurePublished by Informa UK Limited ,2008
- Heavy-Metal and Benzalkonium Chloride Resistance of Listeria monocytogenes Isolates from the Environment of Turkey-Processing PlantsApplied and Environmental Microbiology, 2008
- Teichoic Acid Glycosylation Mediated by gtcA Is Required for Phage Adsorption and Susceptibility of Listeria monocytogenes Serotype 4bApplied and Environmental Microbiology, 2008
- Incidence and Control of Listeria in Food Processing FacilitiesPublished by Informa UK Limited ,2007
- Serological and molecular ecology of Listeria monocytogenes isolates collected from 13 French pork meat salting–curing plants and their productsInternational Journal of Food Microbiology, 2006
- Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in FloridaApplied and Environmental Microbiology, 2006
- Molecular Characterization ofListeria monocytogenesof the Serotype 4b Complex (4b, 4d, 4e) from Two Turkey Processing PlantsFoodborne Pathogens & Disease, 2005
- Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCRJournal of Clinical Microbiology, 2004
- Listeria monocytogenes in poultry and poultry products: epidemiological investigations in seven Danish abattoirsJournal of Applied Bacteriology, 1996
- Phage typing of Listeria monocytogenesInternational Journal of Food Microbiology, 1989