Flow microfluorometric analysis of living spermatozoa stained with Hoechst 33342

Abstract

Bovine spermatozoa were stained with Hoechst 33,342. The fluorescence distribution of stained spermatozoa was complex. Non-motile spermatozoa displayed a higher fluorescence than did motile spermatozoa. The fluorescence profile of the motile spermatozoa was bimodal. Sort and re-analysis, and orientation experiments suggested that there are 2 distinct populations of motile spermatozoa.