Cholecystokinin in White Sea Bream: Molecular Cloning, Regional Expression, and Immunohistochemical Localization in the Gut after Feeding and Fasting
Open Access
- 20 December 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (12), e52428
- https://doi.org/10.1371/journal.pone.0052428
Abstract
The peptide hormone cholecystokinin (CCK), secreted by the midgut, plays a key role in digestive physiology of vertebrates including teleosts, by stimulating pancreatic secretion, gut motility, and gallbladder contraction, as well as by delaying gastric emptying. Moreover, CCK is involved in the regulation of food intake and satiation. Secretion of CCK by the hindgut is controversial, and its biological activity remains to be elucidated. The present paper addresses the regional distribution of intestinal CCK in the white sea bream, Diplodus sargus, as well as the possible involvement of hindgut CCK in digestive processes. Full-lengths mRNAs encoding two CCK isoforms (CCK-1 and CCK-2) were sequenced and phylogenetically analyzed. CCK gene and protein expression levels in the different gut segments were measured 3 h and 72 h after feeding, by quantitative real-time RT-PCR and Western blot, respectively. Moreover, endocrine CCK cells were immunoistochemically detected. Fasting induced a significant decrease in CCK-2 in all intestinal segments, including the hindgut. On the other hand, no significant difference was induced by fasting on hindgut CCK-1. The results demonstrated two CCK isoforms in the hindgut of D.sargus, one of which (CCK-2) may be involved in the feedback control of uncompleted digestive processes. On the other hand, a functional role alternative to regulation of digestive processes may be inferred for D.sargus CCK-1, since its expression was unaffected by feeding or fasting.Keywords
This publication has 41 references indexed in Scilit:
- Gut peptides in the control of food intakeInternational Journal of Obesity, 2009
- Mechanisms of CCK signaling from gut to brainCurrent Opinion in Pharmacology, 2007
- Digestive physiology of marine fish larvae: Hormonal control and processing capacity for proteins, peptides and amino acidsAquaculture, 2007
- How does cholecystokinin stimulate exocrine pancreatic secretion? From birds, rodents, to humansAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2007
- Gastrointestinal hormones regulating appetitePhilosophical Transactions Of The Royal Society B-Biological Sciences, 2006
- Selection method of new candidates for finfish aquaculture: the case of the French Atlantic, the Channel and the North Sea coastsAquatic Living Resources, 2002
- Ontogeny of Cholecystokinin-Immunoreactive Cells in the Digestive Tract of Atlantic Halibut, Hippoglossus hippoglossus, LarvaeGeneral and Comparative Endocrinology, 2001
- Development of Cholecystokinin and Pancreatic Polypeptide Endocrine Systems during the Larval Stage of Japanese Flounder, Paralichthys olivaceusGeneral and Comparative Endocrinology, 2000
- The rapid generation of mutation data matrices from protein sequencesBioinformatics, 1992
- A comparative immunocytochemical study of the gastro-entero-pancreatic (GEP) endocrine system in a stomachless and a stomach-containing teleostGeneral and Comparative Endocrinology, 1987