S1-hypersensitive sites in eukaryotic promoter regions
Open Access
- 12 November 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (21), 8043-8058
- https://doi.org/10.1093/nar/12.21.8043
Abstract
We have examined by fine mapping the S1 nuclease-hypersensitivity of the 5′ flanking regions of the human β-globin and rat preproinsulin II genes and of the SV40 origin/enhancer region. In all cases S1-hypersensitive sites are located in known or presumed promoter/regulatory regions. Though a consensus DNA sequence is not evident, all of these sites reside in predominantly homopurine-homopyrimidine stretches. The alternate (non-B) DNA structure which is revealed by the enzymatic probe is a sequence-dependent feature of a short stretch of DNA, which is retained upon transplantation into a foreign environment. The alternate structure exhibits S1-nicking patterns uniquely different from those associated with the presence of Z-DNA.Keywords
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