Antinociceptive and anti-inflammatory activities ofBunium persicumessential oil, hydroalcoholic and polyphenolic extracts in animal models

Abstract

Fruits of Bunium persicum (Boiss.) B. Fedtsh. (Apiaceae) are widely used in Iranian folkloric medicine for controlling colic pain and dysmenorrhea. This study was aimed to evaluate the anti-inflammatory and analgesic effects of the plant fruits and analyzing its essential oil. Hydroalcoholic extract, polyphenolic extract and essential oil were prepared using standard methods. The acetic acid and formalin tests were used in male mice (25-35 g) to assess analgesic activity. For evaluation of anti-inflammatory effect, carrageenan-induced rat paw edema and croton oil-induced ear edema were used. Hydrodistillation of the fruits of B. persicum afforded a pale yellowish oil in a yield of 2%. GC/MS analysis identified 10 compounds, and gamma-terpinene (46.1%), cuminal (23.9%) and p-cymene (15.9%) were the main components. Hydroalcoholic and polyphenolic extracts (400 and 800 mg/kg, i.p.) and essential oil (100-400 µl/kg, p.o.) significantly (p < 0.01) reduced acetic acid-induced writhings. They also significantly reduced the pain response of both early and late phases of the formalin test. In the carrageenan test and croton oil-induced ear edema both extracts and essential oil showed considerable anti-inflammatory response. These results clearly show the analgesic and anti-inflammatory effects of the plant fruits, and since extracts and essential oil relieved the pain of the first phase of the formalin test, it seems that at least a part of analgesic activity is mediated centrally. Meanwhile, the effects observed in this study provide evidence for folkloric uses of the plant fruits in painful and inflammatory conditions.