Studies upon Calcification in vitro

Abstract

Calcification of cartilage and bone from rachitic rats and 2 human cases in vitro was regularly observed in 9 hrs. when the solution contained 10 mgm. Ca and 4 mgm. inorganic P. It occurred most rapidly when the concentration of bone-forming elements and the reaction approached those of normal serum. It was delayed by additions of egg albumin, and completely inhibited by heat, cold, and protoplasmic poisons. It is concluded that failure of calcification of bone and cartilage in rickets is due to an insufficient concentration of the necessary elements in the fluids bathing the tissues.