Solvent-Free Synthesis of Flavour Esters through Immobilized Lipase Mediated Transesterification
Open Access
- 30 May 2013
- journal article
- research article
- Published by Hindawi Limited in Enzyme Research
- Vol. 2013, 1-6
- https://doi.org/10.1155/2013/367410
Abstract
The synthesis of methyl butyrate and octyl acetate through immobilizedRhizopus oryzaeNRRL 3562 lipase mediated transesterification was studied under solvent-free conditions. The effect of different transesterification variables, namely, molarity of alcohol, reaction time, temperature, agitation, addition of water, and enzyme amount on molar conversion (%) was investigated. A maximum molar conversion of 70.42% and 92.35% was obtained in a reaction time of 14 and 12 h with the transesterification variables of 0.6 M methanol in vinyl butyrate and 2 M octanol in vinyl acetate using 80 U and 60 U immobilized lipase with the agitation speed of 200 rpm and 0.2% water addition at 32°C and 36°C for methyl butyrate and octyl acetate, respectively. The immobilized enzyme has retained good relative activity (more than 95%) up to five and six recycles for methyl butyrate and octyl acetate, respectively. Hence, the present investigation makes a great impingement in natural flavour industry by introducing products synthesized under solvent-free conditions to the flavour market.Keywords
This publication has 33 references indexed in Scilit:
- Evolutionary and swarm intelligence‐based approaches for optimization of lipase extraction from fermented brothEngineering in Life Sciences, 2010
- Comparative study of thermostabilty and ester synthesis ability of free and immobilized lipases on cross linked silica gelBioprocess and Biosystems Engineering, 2007
- Lipase catalyzed synthesis of benzyl acetate in solvent-free medium using vinyl acetate as acyl donorBioorganic & Medicinal Chemistry Letters, 2006
- Esterification in organic solvents by lipase immobilized in polymer of PVA–alginate–boric acidProcess Biochemistry, 2005
- An artificial neural network analysis of porcine pancreas lipase catalysed esterification of anthranilic acid with methanolProcess Biochemistry, 2005
- The production of isoamyl acetate using immobilized lipases in a solvent-free systemProcess Biochemistry, 2002
- Lipase-catalyzed synthesis of isoamyl butyrateBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2001
- Lipase Mediated Synthesis of Low Molecular Weight Flavor EstersJournal of Food Science, 1990
- Short chain flavour esters synthesis by microbial lipasesBiotechnology Letters, 1990
- Microbiological and Enzymatic Production of Flavor and Fragrance ChemicalsCritical Reviews in Biotechnology, 1989