Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM
Open Access
- 19 September 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (9), e44409
- https://doi.org/10.1371/journal.pone.0044409
Abstract
The human gastrointestinal tract can be positively modulated by dietary supplementation of probiotic bacteria in combination with prebiotic carbohydrates. Here differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β- linked galactosides and glucosides. These oligosaccharides induced genes encoding phosphoenolpyruvate-dependent sugar phosphotransferase systems (PTS), galactoside pentose hexuronide (GPH) permease, and ATP-binding cassette (ABC) transporters. PTS systems were upregulated primarily by di- and tri-saccharides such as cellobiose, isomaltose, isomaltulose, panose and gentiobiose, while ABC transporters were upregulated by raffinose, Polydextrose, and stachyose. A single GPH transporter was induced by lactitol and galactooligosaccharides (GOS). The various transporters were associated with a number of glycoside hydrolases from families 1, 2, 4, 13, 32, 36, 42, and 65, involved in the catabolism of various α- and β-linked glucosides and galactosides. Further subfamily specialization was also observed for different PTS-associated GH1 6-phospho-β-glucosidases implicated in the catabolism of gentiobiose and cellobiose. These findings highlight the broad oligosaccharide metabolic repertoire of L. acidophilus NCFM and establish a platform for selection and screening of both probiotic bacteria and prebiotic compounds that may positively influence the gastrointestinal microbiota.Keywords
This publication has 55 references indexed in Scilit:
- Barcoded Pyrosequencing Reveals That Consumption of Galactooligosaccharides Results in a Highly Specific Bifidogenic Response in HumansPLOS ONE, 2011
- Probiotics and prebiotics: clinical effects in allergic diseaseCurrent Opinion in Pediatrics, 2010
- The molecular basis of glycogen breakdown and transport in Streptococcus pneumoniaeMolecular Microbiology, 2010
- A Catalog of Reference Genomes from the Human MicrobiomeScience, 2010
- The PTS transporters of Lactobacillus gasseri ATCC 33323BMC Microbiology, 2010
- Some putative prebiotics increase the severity of Salmonella entericaserovar Typhimurium infection in miceBMC Microbiology, 2009
- The maltodextrin transport system and metabolism in Lactobacillus acidophilus NCFM and production of novel α‐glucosides through reverse phosphorolysis by maltose phosphorylaseThe FEBS Journal, 2009
- Probiotic Effects on Cold and Influenza-Like Symptom Incidence and Duration in ChildrenPediatrics, 2009
- Panose, a new prebiotic candidateLetters in Applied Microbiology, 2009
- Comparative genomics of lactic acid bacteria reveals a niche-specific gene setBMC Microbiology, 2009