Chromatin structure of erythroid-specific genes of immature and mature chicken erythrocytes
- 1 October 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 263 (1), 179-186
- https://doi.org/10.1042/bj2630179
Abstract
The .beta.-globin and histone H5 genes are transcriptionally active in immature chicken erythrocytes and potentially active in mature erythrocytes. In both immature and mature erythrocytes, the majority of these erythroid-specific gene sequences are located in two chromatin fractions: the low-salt-insoluble residual nuclear material and the 0.15 M-NaCl-soluble oligo- and poly-nucleosomes. These salt-soluble chromatin fragments are enriched in hyperacetylated species of H4 and H2B, ubiquitinated and polyubiquitinated species of H2A and H2B and are depleted of linker histones H1 and H5. The competent, transcriptionally inactive embryonic .epsilon.-globin gene, which is part of the DNAase I-sensitive .beta.-globin domain, is highly enriched in the 0.15 M-NaCl-soluble polynucleosomes fraction but not in the insoluble nuclear material. The repressed vitellogenin gene shows no enrichment in either of these fractions. These results suggest that only those genes that are expressed or have the potential for expression are enriched in the low-salt-insoluble nuclear material of immature or mature erythrocytes. The enrichment of active genes in the low-salt-insoluble residual nuclear material of immature erythrocytes is not dependent on on-going transcription, the presence of RNA or changes in the amount of acetylated histone species. Our results are consistent with the hypothesis that active and potentially active genes are insoluble because of the presence of preinitiation transcription complexes.This publication has 47 references indexed in Scilit:
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