Evaluation of Six Immunoassays for Detection of Dengue Virus-Specific Immunoglobulin M and G Antibodies
- 1 November 2000
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 7 (6), 867-871
- https://doi.org/10.1128/cdli.7.6.867-871.2000
Abstract
The performance of six commercially available immunoassay systems for the detection of dengue virus-specific immunoglobulin M (IgM) and IgG antibodies in serum was evaluated. These included two IgM and IgG enzyme immunoassays (EIA) from MRL Laboratories and PanBio, a rapid immunochromatographic test (RIT) from PanBio, immunofluorescence assays (IFA) from Progen, a dot blot assay from Genelabs, and a dipstick EIA from Integrated Diagnostics (INDX). For this study a panel of 132 serum samples, including 90 serum samples from patients with suspected dengue virus infection and 42 serum samples from patients with other viral infections, was used. In addition, serial serum samples from two monkeys experimentally immunized and challenged with dengue virus type 2 were used. Results were considered conclusive when concordant results were obtained with four of the six antibody-specific assays. Based on this definition, the calculated overall agreement for the human serum samples for the respective IgM immunoassays was 97% (128 of 132), with 34% (45 of 132) positive serum samples, 63% (83 of 132) negative samples, and 3% of samples (4 of 132) showing discordant results. The calculated overall agreement for the IgG assays was 94% (124 of 132), with 49% (65 of 132) positive, 45% (59 of 132) negative, and 6% (8 of 132) discordant results, respectively. The sensitivities of the dengue virus-specific assays evaluated varied between 71 and 100% for IgM and between 52 and 100% for IgG, with specificities of 86 to 96% and 81 to 100%, respectively. The relative sensitivities of the respective IgM assays measured with the monkey serum samples were comparable with those obtained with 12 serial serum samples from humans. Overall performance, based on the sum of the agreement, sensitivity, specificity, and Kappa statistics of the IgM and IgG immunoassays, showed that the antibody detection systems from INDX and Genelabs and the MRL and PanBio EIA are useful and reliable assays for dengue virus serodiagnosis.Keywords
This publication has 9 references indexed in Scilit:
- Induction of protective immunity against Dengue virus type 2: comparison of candidate live attenuated and recombinant vaccinesVaccine, 1999
- Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infectionClinical and Diagnostic Virology, 1998
- The micro-focus reduction neutralization test for determining dengue and Japanese encephalitis neutralizing antibodies in volunteers vaccinated against dengueTransactions of the Royal Society of Tropical Medicine and Hygiene, 1997
- An evaluation of nine commercial EIA kits for the detection of measles specific IgGJournal of Virological Methods, 1997
- Multicentre evaluation of dengue IgM dot enzyme immunoassayClinical and Diagnostic Virology, 1996
- Protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cellsArchiv für die gesamte Virusforschung, 1994
- Dengue and dengue hemorrhagic feverThe Pediatric Infectious Disease Journal, 1992
- Clinical Validation of Immunoassays: A Well-Designed Approach to a Clinical StudyPublished by Elsevier BV ,1987
- Techniques for Hemagglutination and Hemagglutination-Inhibition with Arthropod-Borne VirusesThe American Journal of Tropical Medicine and Hygiene, 1958