KINETIC EVALUATION OF THE POOL SIZES AND PROLIFERATIVE RESPONSE OF NEUTROPHILS IN BACTERIALLY CHALLENGED AGING MICE

Abstract

Clinical observations during infection suggest that in aged patients, the kinetic or proliferative responses of neutrophils to infection may be deranged. To test this hypothesis, the neutrophil responses of 6-month-old and 30-month-old mice were compared. After intrapulmonary injection of Escherichia coli, young mice exhibited neutrophilia and diminution of the neutrophil storage pool (NSP) by a mean of 6.4 .times. 106 neutrophils/two femurs. This was accompanied by an increase in the pool of CFU-GM from a control value of 1.1 .times. 105 cells/two femurs (range 0.7 to 1.4) to 1.5 .times. 105 (1.1 to 1.9) (P < .05) and the thymidine suicide (relative proliferative rate) of CFU-GM rose from 27% (19 to 42) to 51% (31 to 61) (P < .05). Furthermore, the CFU-GM of infected young mice displayed enhanced differentiation to the neutrophil series. In contrast, old mice exhibited a greater mean diminution of the NSP: 12.8 .times. 106 neutrophils. Also, old mice experienced a reduction in CFU-GM from 2.3 .times. 105 (1.0 to 3.9) (controls) to 1.3 .times. 105 (1.2 to 1.5)/two femurs (P < .05), a reduction in the proliferation of CFU-GM and reduced differentiation of CFU-GM to neutrophils. These experiments establish that the neutrophil response of infected old mice is disordered, with exaggerated depletion of the NSP and lack of stimulus-driven granulocytopoiesis as reflected by a paradoxical reduction in the number and proliferative rate of precursors. This defect may be compounded by decreased differentiation of precursors to neutrophils.