Determination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (5), 1195-1204
- https://doi.org/10.1093/nar/7.5.1195
Abstract
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.Keywords
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