Comparison of chicken 7SK and U6 RNA polymerase III promoters for short hairpin RNA expression
Open Access
- 1 January 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Biotechnology
- Vol. 7 (1), 79
- https://doi.org/10.1186/1472-6750-7-79
Abstract
RNA polymerase III (pol III) type 3 promoters such as U6 or 7SK are commonly used to express short-hairpin RNA (shRNA) effectors for RNA interference (RNAi). To extend the use of RNAi for studies of development using the chicken as a model system, we have developed a system for expressing shRNAs using the chicken 7SK (ch7SK) promoter.Keywords
This publication has 32 references indexed in Scilit:
- Approaches for chemically synthesized siRNA and vector‐mediated RNAiFEBS Letters, 2005
- Recruitment of RNA polymerase III to its target promotersGenes & Development, 2002
- A System for Stable Expression of Short Interfering RNAs in Mammalian CellsScience, 2002
- Role for a bidentate ribonuclease in the initiation step of RNA interferenceNature, 2001
- RNA interference is mediated by 21- and 22-nucleotide RNAsGenes & Development, 2001
- An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cellsNature, 2000
- Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegansNature, 1998
- Identification of a SPH element in the distal region of a human U6 small nuclear RNA gene promoter and characterization of the SPH binding factor in HeLa cell extracts.1996
- Functional characterization of elements in a human U6 small nuclear RNA gene distal control region.Molecular and Cellular Biology, 1993
- Purified octamer binding transcription factors stimulate RNA polymerase III-mediated transcription of the 7SK RNA geneCell, 1989