Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP‐1 family through heterologous expression in yeast
Open Access
- 5 September 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 351 (2), 286-290
- https://doi.org/10.1016/0014-5793(94)00879-5
Abstract
Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (uppressor of + transport growth efect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N‐ethylmaleimide‐sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP‐1). The SKD1 protein constitutes a novel member of this family with 49–58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.Keywords
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