Complementation of a Herpes Simplex Virus Type 1 Vmw110 Deletion Mutant by Human Cytomegalovirus
- 28 February 1989
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 70 (3), 695-704
- https://doi.org/10.1099/0022-1317-70-3-695
Abstract
The herpes simplex virus type 1 (HSV-1) mutant d1403 contains a 2 kb deletion within the sequences encoding the immediate early polypeptide Vmw110. Previous experiments showed that although dl1403 exhibits normal patterns of gene expression following infection at an m.o.i. of 5 p.f.u./cell its growth and plaquing efficiency are impaired in low multiplicity infections, particularly in human fetal lung (HFL) cells. We have now investigated the ability of two other human herpesviruses, varicella-zoster virus (VZV) and human cytomegalovirus (HCMV), to compensate for this defect at low m.o.i. in HLF cells. Co-infection with HCMV resulted in greatly increased plaque numbers and the apparent particle/p.f.u. ratios of dl1403 stocks were reduced to values similar to those exhibited by wild-type HSV-1 stocks. Complementation of dl1403 in low multiplicity infections by HCMV and VZV was also demonstrated by an increased yield of the mutant virus and an increase in synthesis of dl1403 DNA. Ultraviolet irradiation of HCMV abolished its ability to complement dl1403 and the presence of adenovirus 5 had no stimulatory effect on dl1403 DNA replication. When HFL monolayers were infected with dilutions of dl1403 stocks such that no plaques were produced, replication of the mutant virus could be induced by superinfection with HCMV 7 days after the initial infection. These results indicate that a non-lytic interaction between dl1403 and HFL cells is a more likely consequence of a low multiplicity infection than plaque formation.This publication has 7 references indexed in Scilit:
- Cell lines containing varicella-zoster virus open reading frame 62 and expressing the "IE" 175 protein complement ICP4 mutants of herpes simplex virus type 1Journal of Virology, 1988
- Differences in Cell Type-specific Blocks to Immediate Early Gene Expression and DNA Replication of Human, Simian and Murine CytomegalovirusJournal of General Virology, 1988
- Characterization of the IE110 Gene of Herpes Simplex Virus Type 1Journal of General Virology, 1986
- Identification of a Varicella-Zoster Virus Origin of DNA Replication and Its Activation by Herpes Simplex Virus Type 1 Gene ProductsJournal of General Virology, 1986
- Herpes simplex virus type 1 ICP27 is an essential regulatory proteinJournal of Virology, 1985
- Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptidesVirology, 1982