Prostaglandin E2 is a negative regulator on human plasmacytoid dendritic cells

Abstract

Prostaglandin E₂ (PGE₂), a major lipid derived from the metabolism of arachidonic acid, is an environmentally bioactive substance produced by inflammatory processes and acts as a cAMP up‐regulator that plays an important role in immune responses. It has been reported that PGE₂ has the ability to inhibit the production of interleukin‐12 by myeloid dendritic cells (MDCs) and macrophages, and then induce preferential T helper type 2 (Th2) cell responses. However, little is known of the function of PGE₂ for plasmacytoid dendritic cells (PDCs), which may contribute to the innate and adaptive immune response to viral infection, allergy and autoimmune diseases. In the present study, we compared the biological effect of PGE₂ on human PDCs and MDCs. PGE₂ caused the death of PDCs but MDCs survived. Furthermore, we found that, whereas PGE₂ inhibited interferon‐α production by PDCs in response to virus or cytosine–phosphate–guanosine, it inhibited interelukin‐12 production by MDCs in response to lipopolysaccharide (LPS) or poly(I:C). Although both virus‐stimulated PDCs and LPS‐stimulated MDCs preferentially induced the development of interferon‐γ‐producing Th1 cells, pretreatment with PGE₂ led both DC subsets to attenuate their Th1‐inducing capacity. These findings suggest that PGE₂ represents a negative regulator on not only MDCs but also PDCs.