Visualizing of the cellular uptake and intracellular trafficking of exosomes by live‐cell microscopy
- 7 June 2010
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 111 (2), 488-496
- https://doi.org/10.1002/jcb.22733
Abstract
Cells release exosomes to transfer various molecules to other cells. Exosomes are involved in a number of physiological and pathological processes. They are emerging great potential utility for diseases diagnosis and treatment recently. However, the internalization and intracellular trafficking of exosomes have not been described clearly. In this work, exosomes were isolated from the culture medium of PC12 cells, labeled by lipophilic dye and amino‐reactive fluorophore, incubated with resting PC12 cells. The results of live‐cell microscopy indicated that exosomes were internalized through endocytosis pathway, trapped in vesicles, and transported to perinuclear region. Particle tracking fluorescent vesicles suggested that the active transport of exosomes may be mediated by cytoskeleton. The proteins on exosome membrane were found to be released from exosomes and trapped in lysosome. The inverted transport of lipophilic dye from perinuclear region to cell peripheries was revealed, possibly caused by recycling of the exosome lipids. This study provides new sight into the mechanisms of exosome uptake and intracellular fate. J. Cell. Biochem. 111: 488–496, 2010.Keywords
This publication has 30 references indexed in Scilit:
- Cellular Internalization of Exosomes Occurs Through PhagocytosisTraffic, 2010
- Microenvironmental pH Is a Key Factor for Exosome Traffic in Tumor CellsJournal of Biological Chemistry, 2009
- Active Uptake of Dendritic Cell-Derived Exovesicles by Epithelial Cells Induces the Release of Inflammatory Mediators through a TNF-α-Mediated PathwayThe American Journal of Pathology, 2009
- Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkersNature, 2008
- Endocytic Trafficking of Sphingomyelin Depends on Its Acyl Chain LengthMolecular Biology of the Cell, 2007
- Precise particle tracking against a complicated background: polynomial fitting with Gaussian weightPhysical Biology, 2007
- Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cellsNature, 2007
- Exosomal transfer of proteins and RNAs at synapses in the nervous systemBiology Direct, 2007
- Exovesicles from Human Activated Dendritic Cells Fuse with Resting Dendritic Cells, Allowing Them to Present AlloantigensThe American Journal of Pathology, 2006
- Dynein structure and power strokeNature, 2003