Crystal structure of a GA protein βγdimer at 2.1 Å resolution
- 25 January 1996
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 379 (6563), 369-374
- https://doi.org/10.1038/379369a0
Abstract
Many signalling cascades use seven-helical transmembrane receptors coupled to heterotrimeric G proteins (G alpha beta gamma) to convert extracellular signals into intracellular responses. Upon nucleotide exchange catalysed by activated receptors, heterotrimers dissociate into GTP-bound G alpha subunits and G beta gamma dimers, either of which can modulate many downstream effectors. Here we use multiwavelength anomalous diffraction data to solve the crystal structure of the beta gamma dimer of the G protein transducin. The beta-subunit is primarily a seven-bladed beta-propeller that is partially encircled by an extended gamma-subunit. The beta-propeller, which contains seven structurally similar WD repeats, defines the stereochemistry of the WD repeat and the probable architecture of all WD-repeat-containing domains. The structure details interactions between G protein beta- and gamma-subunits and highlights regions implicated in effector modulation for the conserved family of G protein beta gamma dimers.Keywords
This publication has 32 references indexed in Scilit:
- Structural and functional relationships of heterotrimeric G‐proteinsThe FASEB Journal, 1995
- The ancient regulatory-protein family of WD-repeat proteinsNature, 1994
- New roles for G-protein (βγ-dimers in transmembrane signallingNature, 1993
- G proteinsTrends in Biochemical Sciences, 1992
- Structural principles for the propeller assembly of β‐sheets: The preference for seven‐fold symmetryProteins-Structure Function and Bioinformatics, 1992
- The WD‐40 repeatFEBS Letters, 1992
- Comparative analysis of the β transducin family with identification of several new members including PWP1, a nonessential gene of Saccharomyces cerevisiae that is divergently transcribed from NMT1Proteins-Structure Function and Bioinformatics, 1992
- Novel thioether bond revealed by a 1.7 Å crystal structure of galactose oxidaseNature, 1991
- Pheromone-induced phosphorylation of a G protein β subunit in S. cerevisiae is associated with an adaptive response to mating pheromoneCell, 1991
- Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs.Proceedings of the National Academy of Sciences, 1986