MicroRNA-613 represses lipogenesis in HepG2 cells by downregulating LXRα
Open Access
- 8 March 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Lipids in Health and Disease
- Vol. 12 (1), 32
- https://doi.org/10.1186/1476-511x-12-32
Abstract
MicroRNAs (miRNAs) emerge as new important regulators of lipid homeostasis by regulating corresponding genes. MiR-613 is a newly discovered microRNA, of which the biological function is unknown. A recent report has shown that miR-613 downregulates liver X receptor α (LXRα), a ligand-activated nuclear receptor playing an important role in the regulation of lipid metabolism. The purpose of this study is to explore the effect and the molecular basis of miR-613 on lipogenesis in HepG2 cells. HepG2 cells were transiently transfected with miR-613 mimic or control microRNA. Real time PCR, Western blot, Luciferase reporter assay and Oil Red O staining were employed to examine the expression of LXRα and its target genes involved in lipogenesis, binding site for miR-613 in 3′-untranslated region (3′-UTR) of LXRα mRNA and lipid droplet accumulation in the cells. MiR-613 dramatically suppressed the expression of LXRα and its target genes including sterol-regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), carbohydrate responsive element-binding protein (ChREBP) and acetyl-CoA carboxylase (ACC). Reporter assay showed that miR-613 directly bound to 3′-UTR of LXRα mRNA. Moreover, miR-613 significantly repressed LXRα-induced lipid droplet accumulation in HepG2 cells. Ectopic expression of LXRα without 3′-UTR markedly attenuated the miR-613-mediated downregulation of LXRα’s target genes and LXRα-induced lipid droplet accumulation. MiR-613 suppresses lipogenesis by directly targeting LXRα in HepG2 cells, suggesting that miR-613 may serve as a novel target for regulating lipid homeostasis.Keywords
This publication has 27 references indexed in Scilit:
- A Systematic Screen for Micro-RNAs Regulating the Canonical Wnt PathwayPLOS ONE, 2011
- MicroRNA involvement in esophageal carcinogenesisCurrent Opinion in Pharmacology, 2011
- The Role of MicroRNAs in Cholesterol Efflux and Hepatic Lipid MetabolismAnnual Review of Nutrition, 2011
- Kaposi's sarcoma‐associated herpesviral IL‐6 and human IL‐6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulationThe Journal of Pathology, 2011
- MicroRNA hsa-miR-613 Targets the Human LXRα Gene and Mediates a Feedback Loop of LXRα AutoregulationMolecular Endocrinology, 2011
- MicroRNAs in lipid metabolismCurrent Opinion in Lipidology, 2011
- Translational Regulation of Gene Expression during Conditions of Cell StressMolecular Cell, 2010
- MicroRNA and vascular smooth muscle cell phenotype: new therapy for atherosclerosis?Genome Medicine, 2009
- Phosphorylation of Liver X Receptor α Selectively Regulates Target Gene Expression in MacrophagesMolecular and Cellular Biology, 2008
- Obesity and insulin resistanceJCI Insight, 2000